Journal: Frontiers in Immunology

Article Title: Establishment and validation of evaluation models for post-inflammatory pigmentation abnormalities

doi: 10.3389/fimmu.2022.991594

Figure Lengend Snippet: The role of IL-37 in melanogenesis. MNT1 cells were treated with different concentrations of IL-37. (A) qRT-PCR was used to examine the mRNA levels of melanogenesis-related genes. (B) The protein levels of MITF, TYR, TYRP1, DCT, PMEL17 and MLANA were analyzed by Western blotting. (C) Tyrosinase activity was measured by L-DOPA assay. (D) representative immunofluorescence images showing the distribution of melanosomes. (N = 3, ANOVA, error bar represents mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001).

Article Snippet: Primary antibodies specific for GAPDH (#AP0066, Bioworld), TYR (BS1484, Bioworld), MITF (STJ94134, St. John’s Laboratory), TYRP1 (ab235447, Abcam), DCT (NBP1-56058, Novusbio), PMEL17(#H1219, Santa Cruz and bs-17478R, Bioss) and MLANA (bs-0051R, Bioss and ET1610-47, HUABIO) were purchased as indicated.

Techniques: Quantitative RT-PCR, Western Blot, Activity Assay, Immunofluorescence

Journal: Immunity

Article Title: Clonal Deletion of Tumor-Specific T Cells by Interferon-γ Confers Therapeutic Resistance to Combination Immune Checkpoint Blockade

doi: 10.1016/j.immuni.2019.01.006

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Sections were then duo-stained with CD8 antibody (Dako Cat. M7103) and MART-1 antibody (Novus Biotechne Cat. NBP1–30151) using the Envision G2 Doublestain Kit (Dako Cat. K5361).

Techniques: In Vivo, Injection, Sequencing, Recombinant, Blocking Assay, Plasmid Preparation, Saline, Lysis, Isolation, Staining, Software

Journal: Cell Death & Disease

Article Title: CXCL12 loaded-dermal filler captures CXCR4 expressing melanoma circulating tumor cells

doi: 10.1038/s41419-019-1796-6

Figure Lengend Snippet: A Cells recovered from EG and CLG gel were seeded on plates immediately after collection. Microphotographs from EG (panel A) and CLG (panel B) at 3 and 7days (Axiovert10 Microscopy-Zeiss). Scale bar is 20 µm. CLG cells express B c-Kit, C Melan A, S100 and D CXCR4 while EG cells express B α-SMA but do not express c-Kit, Melan A and S100. Cells (1 x 10 4 ) were seeded on glass coverslips, fixed with 4% paraformaldehyde (15 min, 4 °C) and stained with rabbit anti-c-Kit primary antibody, mouse anti-αSMA primary antibody, mouse anti-S100 primary antibody mouse, mouse anti-Melan A primary antibody and mouse anti-human CXCR4 primary antibody (clone 12G5 R&D). DAPI was used to stain the cell nucleus (Carlo Zeiss, Axio Scope.A1). CXCR4 expression was compared to PES43, human melanoma cell line-CXCR4 expressing, FB-1, human anaplastic thyroid cancer cell line- low CXCR4 expressing and murine B16-hCXCR4-GFP cells evaluated in the in vitro/in vivo assay. DAPI was used to stain the cell nucleae. Magnification 400X. Scale bar is 25 µm

Article Snippet: Cells (1 x 10 4 ) were seeded on glass coverslips, fixed with 4% paraformaldehyde (15 min, 4 °C) and stained with rabbit anti-c-Kit primary antibody, mouse anti-αSMA primary antibody, mouse anti-S100 primary antibody mouse, mouse anti-Melan A primary antibody and mouse anti-human CXCR4 primary antibody (clone 12G5 R&D).

Techniques: Microscopy, Staining, Expressing, In Vitro, In Vivo